The Ernst Wanke Road intersection will be closed overnight from 8pm Thursday 21 July to 5am Friday 22 July, as part of works on the Narre Warren North Road Upgrade.
The closure will allow crews to move traffic barriers from the side of the road, to the median.
Road signage will be in place, but motorists are also encouraged to take extra care and allow for extra time on their journeys.
It is part of Major Road Projects Victoria’s upgrade to Narre Warren North Road, which will also add extra lanes between Belgrave-Hallam Road and Fox Road and upgrade key intersections throughout Narre Warren North.